Protein Analysis Reagents
Thermo Scientific™ 8M Guanidine-HCl Solution
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
BD Cytofix™ Fixation Buffer
Can be used to fix unstained cells for subsequent immunofluorescent staining of intracellular cytokines. Can also be used to fix cells after immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time.
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ Slide-A-Lyzer™ G3 Dialysis Cassettes, 20K MWCO
Thermo Scientific Slide-A-Lyzer™ G3 Dialysis Cassettes facilitate the rapid and trouble-free dialysis of sample volumes from 0.5 to 125 mL. Unlike standard flat tubing, these innovative devices do not require knots or clips that can lead to leaking and sample loss.
Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ Pierce™ LTQ Velos ESI Positive Ion Calibration Solution
Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.
Thermo Scientific™ Pierce™ BCA Protein Assay Reagent B
Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.
Thermo Scientific™ Pierce™ Bovine Serum Albumin Standard, 2 mg/mL
High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
BD 7-AAD Staining Solution
Convenient, ready-to-use nucleic acid dye that can be used in place of propidium iodide (PI)
Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
BD GolgiPlug™ Protein Transport Inhibitor (containing Brefeldin A)
Enhance the detectability of cytokine-producing cells with immunofluorescent staining and flow cytometric analysis
Thermo Scientific™ Ionic Detergent Compatibility Reagent for Pierce™ 660nm Protein Assay Reagent
Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Thermo Scientific™ Pierce™ Detergent Compatible Bradford Assay Kit
Quickly and reliably measure protein concentration of cell lysates or other samples that contain Triton™ X-100, NP-40 or other detergents using the the Thermo Scientific™ Pierce™ Detergent Compatible Bradford Assay Kit.
Invitrogen™ One Shot™ BL21(DE3) Chemically Competent E. coli
One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.
Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution
Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10%solution.